SOD2 Recombinant antibody, PBS Only (Capture)

SOD2 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240462F8

Cat no : 83519-2-PBS

Synonyms

SODM, MNSOD, Mn SOD, IPOB, IPO B

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Validation Data Gallery

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  IF Staining of HepG2 using 83519-2-RR (same clone as 83519-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SOD2 antibody (83519-2-RR, Clone: 240462F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83519-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83519-2-RR (same clone as 83519-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SOD2 antibody (83519-2-RR, Clone: 240462F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83519-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83519-2-RR (same clone as 83519-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SOD2 Recombinant antibody (83519-2-RR, Clone:240462F8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83519-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00517-1

  Cytometric bead array standard curve of MP00517-1, SOD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83519-2-PBS. Detection antibody: 83519-1-PBS. Standard: Ag21388. Range: 1.56-200 ng/mL.

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  Affinity and Kinetic Characterization of 83519-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83519-2-RR against Human SOD2 were performed. The affinity constant is 2.75 nM.

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Product Information

83519-2-PBS targets SOD2 as part of a matched antibody pair:

MP00517-1: 83519-2-PBS capture and 83519-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SOD2 fusion protein Ag21388
• Full Name: superoxide dismutase 2, mitochondrial
• Calculated Molecular Weight: 25 kDa
• GenBank Accession Number: BC016934
• Gene Symbol: SOD2
• Gene ID (NCBI): 6648
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa. This antibody is specific to SOD2.