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SOD2 Recombinant antibody

SOD2 Recombinant Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, ELISA

Conjugate

Unconjugated

CloneNo.

240462F8

Cat no : 83519-2-RR

Synonyms

IPO B, Mn SOD, MNSOD, SOD2, SODM

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SOD2 Recombinant antibody (83519-2-RR)

    IF Staining of HepG2 using 83519-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SOD2 antibody (83519-2-RR, Clone: 240462F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SOD2 Recombinant antibody (83519-2-RR)

    IF Staining of HeLa using 83519-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SOD2 antibody (83519-2-RR, Clone: 240462F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive IF detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83519-2-RR is SOD2 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SOD2 fusion protein Ag21388
Full Name superoxide dismutase 2, mitochondrial
Calculated Molecular Weight 25 kDa
GenBank Accession NumberBC016934
Gene Symbol SOD2
Gene ID (NCBI) 6648
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa. This antibody is specific to SOD2.

Protocols

Product Specific Protocols
IF protocol for SOD2 antibody 83519-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols