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CoraLite® Plus 488-conjugated SNRPN Monoclonal antibody

SNRPN Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H2F4

Cat no : CL488-67280

Synonyms

SMN, Sm-D, Small nuclear ribonucleoprotein-associated protein N, SM D, HCERN3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using CoraLite® Plus 488-conjugated SNRPN Monoclonal ant (CL488-67280)

    IF Staining of U-251 using CL488-67280

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using CoraLite® Plus 488 SNRPN antibody (CL488-67280, Clone: 1H2F4 ) at dilution of 1:1000, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated SNRPN Monoclonal ant (CL488-67280)

    FC experiment of HeLa using CL488-67280

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SNRPN (CL488-67280, Clone:1H2F4) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67280 targets SNRPN in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SNRPN fusion protein Ag14030
Full Name small nuclear ribonucleoprotein polypeptide N
Calculated Molecular Weight 240 aa, 25 kDa
Observed Molecular Weight 27-29 kDa
GenBank Accession NumberBC000611
Gene Symbol SNRPN
Gene ID (NCBI) 6638
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNRPN, or SNURF-SNRPN, is a bicistronic imprinted gene that encodes 2 polypeptides, the SmN splicing factor, which is involved in RNA processing, and the SNRPN upstream reading frame (SNURF) polypeptide.The SNRPN gene is transcribed exclusively from the paternally inherited chromosome and shows highest expression in brain and heart. SNRPN may play a role in etiology of Prader-Willi syndrome.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SNRPN antibody CL488-67280Download protocol
FC protocol for CL Plus 488 SNRPN antibody CL488-67280Download protocol
Standard Protocols
Click here to view our Standard Protocols