PE-conjugated SNRNP200 Polyclonal antibody

SNRNP200 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

PE Fluorescent Dye

Cat no : PE-23875

Synonyms

KIAA0788, HELIC2, EC:3.6.4.13, BRR2, ASCC3L1

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Validation Data Gallery

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  FC experiment of A431 using PE-23875

  1x10^6 A431 cells were intracellularly stained with 0.2 ug PE Snrnp200 Polyclonal Antibody (PE-23875)(red), or 0.2 ug Control Antibody (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-23875 targets SNRNP200 in FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNRNP200 fusion protein Ag20748
• Full Name: small nuclear ribonucleoprotein 200kDa (U5)
• Calculated Molecular Weight: 494 aa, 57 kDa
• Observed Molecular Weight: 200-245 kDa
• GenBank Accession Number: BC001417
• Gene Symbol: SNRNP200
• Gene ID (NCBI): 23020
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

SNRNP200 (small nuclear ribonucleoprotein 200kDa (U5)), also known as HELIC2, ASCC3L1 or BRR2, is a 2,136 amino acid protein that localizes to the nucleus and contains two SEC63 domains, two helicase C-terminal domains and two helicase ATP-binding domains. Existing as multiple alternatively spliced isoforms, HELIC2 functions as an RNA helicase that is thought to promote specific RNA-RNA conformational changes which are important in the second step of RNA splicing. The gene encoding HELIC2 maps to human chromosome 2, which houses over 1,400 genes and comprises nearly 8% of the human genome. Harlequin icthyosis, a rare and morbid skin deformity, is associated with mutations in the chromosome 2-localized ABCA12 gene, while the lipid metabolic disorder sitosterolemia is associated with defects in the ABCG5 and ABCG8 genes, hich also map to chromosome 2. The observed molecular weight of SNRNP200 is 200-245kd.