SND1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SND1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A6A4

Cat no : 60265-1-PBS

Synonyms

100 kDa coactivator, EBNA2 coactivator p100, p100, p100 co activator, SND1, TDRD11

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Validation Data Gallery

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  WB analysis using 60265-1-Ig (same clone as 60265-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60265-1-Ig (SND1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 60265-1-Ig (same clone as 60265-1-PBS)

  WB result of SND1 antibody (60265-1-Ig, 1:60,000) with si-Control and si-SND1 transfected Jurkat cells. This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

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  WB analysis using 60265-1-Ig (same clone as 60265-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60265-1-Ig (SND1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60265-1-Ig (same clone as 60265-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60265-1-Ig (SND1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 60265-1-Ig (same clone as 60265-1-PBS)

  IP result of anti-SND1 (IP:60265-1-Ig, 5ug; Detection:60265-1-Ig 1:500) with HeLa cells lysate 1400ug. This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60265-1-Ig (same clone as 60265-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60265-1-Ig (SND1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 60265-1-Ig (same clone as 60265-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60265-1-Ig (SND1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60265-1-Ig (same clone as 60265-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60265-1-Ig (SND1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast hyperplasia using 60265-1-Ig (same clone as 60265-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60265-1-Ig (SND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast hyperplasia using 60265-1-Ig (same clone as 60265-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60265-1-Ig (SND1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60265-1-Ig (same clone as 60265-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60265-1-Ig (SND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60265-1-Ig (same clone as 60265-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60265-1-Ig (SND1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 60265-1-Ig (same clone as 60265-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60265-1-Ig (SND1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60265-1-Ig (same clone as 60265-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 60265-1-Ig(SND1 antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60265-1-Ig (same clone as 60265-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SND1 antibody (60265-1-Ig, Clone: 1A6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

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Product Information

60265-1-PBS targets SND1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SND1 fusion protein Ag1200
• Full Name: staphylococcal nuclease and tudor domain containing 1
• Calculated Molecular Weight: 101 kDa
• Observed Molecular Weight: 101 kDa
• GenBank Accession Number: BC017180
• Gene Symbol: SND1
• Gene ID (NCBI): 27044
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Staphylococcal nuclease domain-containing 1 (SND1), is a multifunctional nuclease that consists of four staphylococcal nuclease domains and a tudor domain. SND1 acts as a coactivator that facilitates transcriptional activity of STAT5, 6 and c-Myc. SND1 is a comprising part of the RNA-induced silencing complex(RISC), and takes part in the functions of miRNA, regulates transcription through transcriptional coactivation, RNA interference, RNA splicing, and RNA editing. Higher level of SND1 has been found in colon cancer and prostate cancer, can promote HCC angiogenesis in xenograft model through induction of angiogenic factors.