SND1 Monoclonal antibody

• Featured Product
• KD/KO Validated

SND1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

10

CloneNo.

1A6A4

Cat no : 60265-1-Ig

Synonyms

100 kDa coactivator, EBNA2 coactivator p100, p100, p100 co activator, SND1, TDRD11

---

Validation Data Gallery

• 

  WB analysis using 60265-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60265-1-Ig (SND1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 60265-1-Ig

  WB result of SND1 antibody (60265-1-Ig, 1:60,000) with si-Control and si-SND1 transfected Jurkat cells.

• 

  WB analysis using 60265-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60265-1-Ig (SND1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 60265-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60265-1-Ig (SND1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 60265-1-Ig

  IP result of anti-SND1 (IP:60265-1-Ig, 5ug; Detection:60265-1-Ig 1:500) with HeLa cells lysate 1400ug.

• 

  IHC staining of human pancreas using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60265-1-Ig (SND1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60265-1-Ig (SND1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast hyperplasia using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60265-1-Ig (SND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60265-1-Ig (SND1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60265-1-Ig (SND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60265-1-Ig (SND1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60265-1-Ig (SND1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60265-1-Ig (SND1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 60265-1-Ig

  Immunofluorescent analysis of HepG2 cells using 60265-1-Ig(SND1 antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-mouse IgG (red).

• 

  IF Staining of HepG2 using 60265-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SND1 antibody (60265-1-Ig, Clone: 1A6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

---

Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, Jurkat cells, U2OS cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells, A431 cells, LNCaP cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human pancreas tissue, human breast hyperplasia tissue, human breast cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 5 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 60265-1-Ig is SND1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SND1 fusion protein Ag1200
• Full Name: staphylococcal nuclease and tudor domain containing 1
• Calculated Molecular Weight: 101 kDa
• Observed Molecular Weight: 101 kDa
• GenBank Accession Number: BC017180
• Gene Symbol: SND1
• Gene ID (NCBI): 27044
• RRID: AB_2881386
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Staphylococcal nuclease domain-containing 1 (SND1), is a multifunctional nuclease that consists of four staphylococcal nuclease domains and a tudor domain. SND1 acts as a coactivator that facilitates transcriptional activity of STAT5, 6 and c-Myc. SND1 is a comprising part of the RNA-induced silencing complex(RISC), and takes part in the functions of miRNA, regulates transcription through transcriptional coactivation, RNA interference, RNA splicing, and RNA editing. Higher level of SND1 has been found in colon cancer and prostate cancer, can promote HCC angiogenesis in xenograft model through induction of angiogenic factors.

Publications

Species	Application	Title
human	WB	Cell; SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9; Authors - Nora Schmidt
human	IF	Sci Adv; Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.; Authors - Yuan Wang
human	IF, ELISA	Sci Adv; Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.; Authors - Yuan Wang
human	WB	Elife; The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus.; Authors - Belinda Baquero-Perez ; KD Validated
human	WB	Life Sci Alliance; The nucleolar phase of signal recognition particle assembly; Authors - Amani Issa
	IF	J Cell Physiol; The phosphorylation of Tudor-SN mediated by JNK is involved in the regulation of milk protein synthesis induced by prolactin in BMECs.; Authors - Sen Zhang