CoraLite®594-conjugated SNAP25 Monoclonal antibody

SNAP25 Monoclonal Antibody for WB, IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3E4B7

Cat no : CL594-60159

Synonyms

SNAP 25, SNAP, RIC-4, RIC4, RIC 4

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Validation Data Gallery

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  WB analysis of mouse brain using CL594-60159

  mouse brain tissue were subjected to SDS PAGE followed by western blot with CL594-60159 (SNAP25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-12 using CL594-60159

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CoraLite®594 SNAP25 antibody (CL594-60159, Clone: 3E4B7 ) at dilution of 1:200.

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  IF Staining of PC-12 using CL594-60159

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CL594-60159 (SNAP25 antibody) at dilution of 1:100.

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IF/ICC detected in: PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60159 targets SNAP25 in WB, IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SNAP25 fusion protein Ag6695
• Full Name: synaptosomal-associated protein, 25kDa
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC010647
• Gene Symbol: SNAP25
• Gene ID (NCBI): 6616
• RRID: AB_2919898
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells. In addition, it has been shown that inhibition of neurosecretion by botulinum toxin E can be rescued by a SNAP-25 C-terminal peptide, probably by initiating the formation of a fusion competent SNARE complex.