SNAP25 Monoclonal antibody, PBS Only

SNAP25 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E4B7

Cat no : 60159-1-PBS

Synonyms

RIC 4, RIC4, SEC9, SNAP, SNAP 25, SNAP25, SUP, Super protein

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Validation Data Gallery

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  WB analysis of pig brain using 60159-1-Ig (same clone as 60159-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60159-1-Ig (SNAP25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 60159-1-Ig (same clone as 60159-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60159-1-Ig (SNAP25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 60159-1-Ig (same clone as 60159-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60159-1-Ig (SNAP25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 60159-1-Ig (same clone as 60159-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60159-1-Ig (SNAP25 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 60159-1-Ig (same clone as 60159-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 60159-1-Ig (SNAP25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 60159-1-Ig (same clone as 60159-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60159-1-Ig (SNAP25 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 60159-1-Ig (same clone as 60159-1-PBS)

  IP result of anti-SNAP25 (IP:60159-1-Ig, 3ug; Detection:60159-1-Ig 1:500) with mouse brain tissue lysate 3600ug. This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 60159-1-Ig (same clone as 60159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60159-1-Ig (SNAP25 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 60159-1-Ig (same clone as 60159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60159-1-Ig (SNAP25 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 60159-1-Ig (same clone as 60159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60159-1-Ig (SNAP25 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 60159-1-Ig (same clone as 60159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60159-1-Ig (SNAP25 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-12 using 60159-1-Ig (same clone as 60159-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using SNAP25 antibody (60159-1-Ig, Clone: 3E4B7 ) at dilution of 1:1000 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), SNAP25 antibody (14903-1-AP, green). This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-12 using 60159-1-Ig (same clone as 60159-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using SNAP25 antibody (60159-1-Ig, Clone: 3E4B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

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Product Information

60159-1-PBS targets SNAP25 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SNAP25 fusion protein Ag6695
• Full Name: synaptosomal-associated protein, 25kDa
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC010647
• Gene Symbol: SNAP25
• Gene ID (NCBI): 6616
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells.