CoraLite® Plus 488-conjugated SMO Monoclonal antibody

SMO Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A11A10

Cat no : CL488-66851-2

Synonyms

Gx, Protein Gx, SMO, SMOH, Smoothened homolog

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Validation Data Gallery

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  IF Staining of hTERT-RPE1 using CL488-66851-2

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 SMO antibody (CL488-66851, Clone: 2B10A11 ) at dilution of 1:200, ARL13B antibody (17711-1-AP, red).

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Tested Applications

• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66851-2 targets SMO in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SMO fusion protein Ag25861
• Full Name: smoothened homolog (Drosophila)
• Calculated Molecular Weight: 787 aa, 86 kDa
• GenBank Accession Number: BC009989
• Gene Symbol: SMO
• Gene ID (NCBI): 6608
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays central roles in animal development and stem-cell function. Defects in Hh signalling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.