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CoraLite® Plus 488-conjugated SMO Monoclonal antibody

SMO Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Canine

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B10A11

Cat no : CL488-66851

Synonyms

Gx, Protein Gx, SMO, SMOH, Smoothened homolog

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using CoraLite® Plus 488-conjugated SMO Monoclonal antib (CL488-66851)

    IF Staining of MDCK using CL488-66851

    Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using CL488-66851 (SMO antibody) at dilution of 1:100.

Tested Applications

Positive IF/ICC detected inMDCK cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66851 targets SMO in IF/ICC applications and shows reactivity with Human, Canine samples.

Tested Reactivity Human, Canine
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SMO fusion protein Ag25861
Full Name smoothened homolog (Drosophila)
Calculated Molecular Weight 787 aa, 86 kDa
GenBank Accession NumberBC009989
Gene Symbol SMO
Gene ID (NCBI) 6608
RRIDAB_2883387
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays central roles in animal development and stem-cell function. Defects in Hh signalling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SMO antibody CL488-66851Download protocol
Standard Protocols
Click here to view our Standard Protocols