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SMARCE1 Monoclonal antibody, PBS Only

SMARCE1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F3D6

Cat no : 66182-1-PBS

Synonyms

BAF57, BRG1 associated factor 57, SMARCE1

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using SMARCE1 Monoclonal antibody (66182-1-Ig)

    WB analysis of Jurkat using 66182-1-Ig (same clone as 66182-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66182-1-Ig (SMARCE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66182-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SMARCE1 Monoclonal antibody (66182-1-Ig)

    IHC staining of human breast cancer using 66182-1-Ig (same clone as 66182-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66182-1-Ig (SMARCE1 Antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 66182-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SMARCE1 Monoclonal antibody (66182-1-Ig)

    IHC staining of human breast cancer using 66182-1-Ig (same clone as 66182-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66182-1-Ig (SMARCE1 Antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 66182-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SMARCE1 Monoclonal antibody (66182-1-Ig)

    IF Staining of HEK-293 using 66182-1-Ig (same clone as 66182-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 66182-1-Ig (SMARCE1 antibody) at dilution of 1:50 and FITC-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66182-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66182-1-PBS targets SMARCE1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMARCE1 fusion protein Ag1161
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC007082
Gene Symbol SMARCE1
Gene ID (NCBI) 6605
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Via antagonizing chromatin-mediated transcriptional repression, the SWI/SNF complex in S. cerevisiae and Drosophila is thought to facilitate transcriptional activation of specific genes. The BRG1/brm-associated factors, or SMARCE1, complex in mammals is functionally related to SWI/SNF and consists of 9 to 12 subunits, some of which are homologous to SWI/SNF subunits. It contains a DNA-binding HMG domain and performs multiple biological functions, including DNA-, protein- and chromatin-binding, transcription coactivating activity, chromatin modification, DNA-dependent negative regulation of transcription, regulation of transcription of the RNA polymerase II promoter. In addition, SMARCE1, as an ER (estrogen receptor) subtype-selective modulator, regulates ER mediated transcription in a ligand-dependent manner and also directly binds to AR (androgen receptor) to alter its activity. The calculated molecular weight of SMARCE1 is 47 kDa, but modified SMARCE1 is about 55 kDa.