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SMARCE1 Monoclonal antibody

SMARCE1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F3D6

Cat no : 66182-1-Ig

Synonyms

BAF57, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1, BRG1-associated factor 57, BRG1 associated factor 57, 1F3D6

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using SMARCE1 Monoclonal antibody (66182-1-Ig)

    WB analysis of Jurkat using 66182-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66182-1-Ig (SMARCE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SMARCE1 Monoclonal antibody (66182-1-Ig)

    IHC staining of human breast cancer using 66182-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66182-1-Ig (SMARCE1 Antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SMARCE1 Monoclonal antibody (66182-1-Ig)

    IHC staining of human breast cancer using 66182-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66182-1-Ig (SMARCE1 Antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SMARCE1 Monoclonal antibody (66182-1-Ig)

    IF Staining of HEK-293 using 66182-1-Ig

    Immunofluorescent analysis of HEK-293 cells using 66182-1-Ig (SMARCE1 antibody) at dilution of 1:50 and FITC-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SMARCE1 Monoclonal antibody (66182-1-Ig)

    IF Staining of HEK-293 using 66182-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SMARCE1 antibody (66182-1-Ig, Clone: 1F3D6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66182-1-Ig targets SMARCE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMARCE1 fusion protein Ag1161
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC007082
Gene Symbol SMARCE1
Gene ID (NCBI) 6605
RRIDAB_2881577
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Via antagonizing chromatin-mediated transcriptional repression, the SWI/SNF complex in S. cerevisiae and Drosophila is thought to facilitate transcriptional activation of specific genes. The BRG1/brm-associated factors, or SMARCE1, complex in mammals is functionally related to SWI/SNF and consists of 9 to 12 subunits, some of which are homologous to SWI/SNF subunits. It contains a DNA-binding HMG domain and performs multiple biological functions, including DNA-, protein- and chromatin-binding, transcription coactivating activity, chromatin modification, DNA-dependent negative regulation of transcription, regulation of transcription of the RNA polymerase II promoter. In addition, SMARCE1, as an ER (estrogen receptor) subtype-selective modulator, regulates ER mediated transcription in a ligand-dependent manner and also directly binds to AR (androgen receptor) to alter its activity. The calculated molecular weight of SMARCE1 is 47 kDa, but modified SMARCE1 is about 55 kDa.

Protocols

Product Specific Protocols
WB protocol for SMARCE1 antibody 66182-1-IgDownload protocol
IHC protocol for SMARCE1 antibody 66182-1-IgDownload protocol
IF protocol for SMARCE1 antibody 66182-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols