CoraLite® Plus 488-conjugated SMARCB1 Monoclonal antibody

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  FC experiment of HeLa using CL488-67979

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SMARCB1 (CL488-67979, Clone:1D10A4) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

SMARCB1 Monoclonal Antibody for FC (Intra)

Cat No. CL488-67979

Clone No.1D10A4

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Synonyms

SMARCB1, SNF5, SNF5L1, Snr1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1, 1D10A4, BAF47, BRG1-associated factor 47, hSNF5, hSNFS, INI1, INI-1, RDT, Sfh1p

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Tested Applications

• Positive FC (Intra) detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67979 targets SMARCB1 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SMARCB1 fusion protein Ag28911
• Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: NM_003073
• Gene Symbol: SMARCB1
• Gene ID (NCBI): 6598
• RRID: AB_2923846
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q12824
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SMARCB1 is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor and mutations in it have been associated with malignant rhabdoid tumors. Two transcript variants encoding different isoforms have been found for this gene.