SMAD4 Recombinant antibody, PBS Only (Detector)

SMAD4 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240026D10

Cat no : 83169-2-PBS

Synonyms

MADH4, MAD homolog 4, JIP, hSMAD4, DPC4

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00213-3

  Sandwich ELISA standard curve of MP00213-3, Rat KIM-1/HAVCR1 Monoclonal Matched Antibody Pair - PBS only. 83221-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0597. 83169-2-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

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  Sample test of MP00213-3

  Organs from 2 rats were rinsed with PBS to remove excess blood, chopped into 1-2 mm pieces, homogenized in 5-10 mL of PBS in a tissue homogenizer, and stored at ≤ -80℃ 5 min. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g to remove particulate. Homogenates from rat kideny tissue were assayed for rat KIM-1/HAVCR1 and measured 71.6 pg/mL.

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  Sample test of MP00213-3

  Serum of eight individual healthy rats were measured. The rat KIM-1/HAVCR1 concentration of detected samples was determined to be 478.5 pg/mL with a range of 105.6-1,038.5 pg/mL.

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  Cytometric bead array standard curve of MP00267-1

  Cytometric bead array standard curve of MP00267-1, SMAD4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83169-4-PBS. Detection antibody: 83169-2-PBS. Standard: Ag0299. Range: 0.625-20 ng/mL.

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  Cytometric bead array standard curve of MP00267-3

  Cytometric bead array standard curve of MP00267-3, SMAD4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83169-3-PBS. Detection antibody: 83169-2-PBS. Standard: Ag0299. Range: 0.625-20 ng/mL.

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Product Information

83169-2-PBS targets SMAD4 as part of a matched antibody pair:

MP00213-3: 83221-1-PBS capture and 83169-2-PBS detection (validated in Sandwich ELISA)

MP00267-1: 83169-4-PBS capture and 83169-2-PBS detection (validated in Cytometric bead array)

MP00267-3: 83169-3-PBS capture and 83169-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SMAD4 fusion protein Ag0299
• Full Name: SMAD family member 4
• Calculated Molecular Weight: 60 kDa
• GenBank Accession Number: BC002379
• Gene Symbol: SMAD4
• Gene ID (NCBI): 4089
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.