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Rat KIM-1/HAVCR1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: rat

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 15.6-1000 pg/mL

Cat No : MP00213-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00213-3, Rat KIM-1/HAVCR1 Monoclonal Matched Antibody Pair - PBS only. 83221-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0597. 83169-2-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

    Sandwich ELISA standard curve of MP00213-3

    Sandwich ELISA standard curve of MP00213-3, Rat KIM-1/HAVCR1 Monoclonal Matched Antibody Pair - PBS only. 83221-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0597. 83169-2-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Serum of eight individual healthy rats were measured. The rat KIM-1/HAVCR1 concentration of detected samples was determined to be 478.5 pg/mL with a range of 105.6-1,038.5 pg/mL.

    Sample test of MP00213-3

    Serum of eight individual healthy rats were measured. The rat KIM-1/HAVCR1 concentration of detected samples was determined to be 478.5 pg/mL with a range of 105.6-1,038.5 pg/mL.

  • Organs from 2 rats were rinsed with PBS to remove excess blood, chopped into 1-2 mm pieces, homogenized in 5-10 mL of PBS in a tissue homogenizer, and stored at ≤ -80℃ 5 min. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g to remove particulate. Homogenates from rat kideny tissue were assayed for rat KIM-1/HAVCR1 and measured 71.6 pg/mL.

    Sample test of MP00213-3

    Organs from 2 rats were rinsed with PBS to remove excess blood, chopped into 1-2 mm pieces, homogenized in 5-10 mL of PBS in a tissue homogenizer, and stored at ≤ -80℃ 5 min. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g to remove particulate. Homogenates from rat kideny tissue were assayed for rat KIM-1/HAVCR1 and measured 71.6 pg/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00213-3 targets KIM-1/HAVCR1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 15.6-1000 pg/mL (Sandwich ELISA);

Capture antibody: Rat KIM-1/HAVCR1 Recombinant antibody, PBS Only (Capture) 83221-1-PBS (230528B9). 100 μg. Concentration 1 mgl/ml.

Detection antibody: SMAD4 Recombinant antibody, PBS Only (Detector) 83169-2-PBS (240026D10). 100 μg. Concentration 1 mgl/ml.

Alternative KIM-1/HAVCR1 matched antibody pairs: MP00116-1, MP00116-2, MP00116-3, MP00213-1, MP00213-2

Matched pair protein standard: Eg0597

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83221-1-PBS
Tested Reactivity rat
Immunogen Recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name hepatitis A virus cellular receptor 1
Synonyms HAVCR1, KIM-1, TIM-1, Kim1, KIM 1
GenBank accession numberNM_173149.2
Gene symbol Havcr1
UniprotO54947
Gene ID (NCBI) 286934
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83169-2-PBS
Tested Reactivity human
Immunogen SMAD4 fusion protein Ag0299
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SMAD family member 4
Synonyms MADH4, MAD homolog 4, JIP, hSMAD4, DPC4
GenBank accession numberBC002379
Gene symbol SMAD4
UniprotQ13485
Gene ID (NCBI) 4089
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.