SMAD2 Recombinant antibody, PBS Only (Capture)

SMAD2 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240950G3

Cat no : 83841-2-PBS

Synonyms

JV18 1, JV18, hSMAD2, hMAD-2, hMAD 2

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00791-1

  Sandwich ELISA standard curve of MP00791-1, Human SMAD2 Recombinant Matched Antibody Pair - PBS only. 83841-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3237. 83841-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  Sample test of MP00791-1

  HEK-293T, HeLa, HepG2 and MCF-7 cell lysates were measured. The human SMAD2 concentration of detected samples was determined to be 43.85 ng/mL in HEK-293T cell lysate (based on a 5.5 mg/mL extract load). The human SMAD2 concentration of detected samples was determined to be 33.98 ng/mL in HeLa cell lysate (based on a 7 mg/mL extract load). The human SMAD2 concentration of detected samples was determined to be 24.50 ng/mL in HepG2 cell lysate (based on a 4.0 mg/mL extract load). The human SMAD2 concentration of detected samples was determined to be 12.02 ng/mL in MCF-7 cell lysate (based on a 3.1 mg/mL extract load).

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  Sample test of MP00791-1

  Serum of sixteen individual healthy human donors was measured. The human SMAD2 concentration of detected samples was determined to be 2.17 ng/mL with a range of 0.34 - 8.22 ng/mL.

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  Cytometric bead array standard curve of MP00791-1

  Cytometric bead array standard curve of MP00791-1, SMAD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83841-2-PBS. Detection antibody: 83841-3-PBS. Standard: Ag3237. Range: 0.469-60 ng/mL.

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  Cytometric bead array standard curve of MP00791-3

  Cytometric bead array standard curve of MP00791-3, SMAD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83841-2-PBS. Detection antibody: 83841-1-PBS. Standard: Ag3237. Range: 0.938-60 ng/mL.

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Product Information

83841-2-PBS targets SMAD2 as part of a matched antibody pair:

MP00791-1: 83841-2-PBS capture and 83841-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00791-3: 83841-2-PBS capture and 83841-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SMAD2 fusion protein Ag3237
• Full Name: SMAD family member 2
• Calculated Molecular Weight: 467 aa, 52 kDa
• GenBank Accession Number: BC014840
• Gene Symbol: SMAD2
• Gene ID (NCBI): 4087
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.