SLP76 Monoclonal antibody, PBS Only

SLP76 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4H10B1

Cat no : 66465-1-PBS

Synonyms

LCP2, Lymphocyte cytosolic protein 2, SLP 76, SLP 76 tyrosine phosphoprotein, SLP76

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 66465-1-Ig (same clone as 66465-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66465-1-Ig (SLP76 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66465-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MOLT-4 using 66465-1-Ig (same clone as 66465-1-PBS)

  MOLT-4 cells were subjected to SDS PAGE followed by western blot with 66465-1-Ig (SLP76 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66465-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 66465-1-Ig (same clone as 66465-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66465-1-Ig (SLP76 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66465-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66465-1-Ig (same clone as 66465-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66465-1-Ig (SLP76 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66465-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66465-1-PBS targets SLP76 in WB, IF, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SLP76 fusion protein Ag3432
• Full Name: lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)
• Calculated Molecular Weight: 533 aa, 60 kDa
• Observed Molecular Weight: 76 kDa
• GenBank Accession Number: BC016618
• Gene Symbol: SLP76
• Gene ID (NCBI): 3937
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LCP2, also named as SLP76, is an adapter protein that acts as a substrate of the T cell antigen receptor (TCR)-activated protein tyrosine kinase pathway. It contains an N-terminal leucine Zip motif, a tyrosine-rich domain, a proline-rich domain and a C-terminal SH2 domain. It is constitutively associated with the growth factor receptor-bound protein 2 (Grb2)-related adapter protein 2 (Grap2, a.k.a. GADS) through the interaction of its proline residues with one of the SH3 domains of Grap2. SLP76 associates with growth factor receptor bound protein 2 (Grap2), and is thought to play a role TCR-mediated intracellular signal transduction. SLP76 is also expressed by mast cells, and mast cell activation was markedly impaired in SLP76-deficient mice.