SLP76 Monoclonal antibody

SLP76 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

4H10B1

Cat no : 66465-1-Ig

Synonyms

LCP2, 4H10B1, SLP 76 tyrosine phosphoprotein, SLP 76, SH2 domain-containing leukocyte protein of 76 kDa

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Validation Data Gallery

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  WB analysis of Jurkat using 66465-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66465-1-Ig (SLP76 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MOLT-4 using 66465-1-Ig

  MOLT-4 cells were subjected to SDS PAGE followed by western blot with 66465-1-Ig (SLP76 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 66465-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66465-1-Ig (SLP76 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 66465-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66465-1-Ig (SLP76 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of Jurkat using 66465-1-Ig

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human SLP76 (66465-1-Ig, Clone:4H10B1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, MOLT-4 cells
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66465-1-Ig targets SLP76 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SLP76 fusion protein Ag3432
• Full Name: lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)
• Calculated Molecular Weight: 533 aa, 60 kDa
• Observed Molecular Weight: 76 kDa
• GenBank Accession Number: BC016618
• Gene Symbol: SLP76
• Gene ID (NCBI): 3937
• RRID: AB_2881833
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LCP2, also named as SLP76, is an adapter protein that acts as a substrate of the T cell antigen receptor (TCR)-activated protein tyrosine kinase pathway. It contains an N-terminal leucine Zip motif, a tyrosine-rich domain, a proline-rich domain and a C-terminal SH2 domain. It is constitutively associated with the growth factor receptor-bound protein 2 (Grb2)-related adapter protein 2 (Grap2, a.k.a. GADS) through the interaction of its proline residues with one of the SH3 domains of Grap2. SLP76 associates with growth factor receptor bound protein 2 (Grap2), and is thought to play a role TCR-mediated intracellular signal transduction. SLP76 is also expressed by mast cells, and mast cell activation was markedly impaired in SLP76-deficient mice.