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SLP76 Monoclonal antibody

SLP76 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

4H10B1

Cat no : 66465-1-Ig

Synonyms

LCP2, 4H10B1, SLP 76 tyrosine phosphoprotein, SLP 76, SH2 domain-containing leukocyte protein of 76 kDa

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using SLP76 Monoclonal antibody (66465-1-Ig)

    WB analysis of Jurkat using 66465-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66465-1-Ig (SLP76 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MOLT-4 cells using SLP76 Monoclonal antibody (66465-1-Ig)

    WB analysis of MOLT-4 using 66465-1-Ig

    MOLT-4 cells were subjected to SDS PAGE followed by western blot with 66465-1-Ig (SLP76 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using SLP76 Monoclonal antibody (66465-1-Ig)

    WB analysis of K-562 using 66465-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66465-1-Ig (SLP76 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SLP76 Monoclonal antibody (66465-1-Ig)

    IF Staining of HeLa using 66465-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66465-1-Ig (SLP76 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of Jurkat cells using SLP76 Monoclonal antibody (66465-1-Ig)

    FC experiment of Jurkat using 66465-1-Ig

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human SLP76 (66465-1-Ig, Clone:4H10B1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inJurkat cells, K-562 cells, MOLT-4 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66465-1-Ig targets SLP76 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SLP76 fusion protein Ag3432
Full Name lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)
Calculated Molecular Weight 533 aa, 60 kDa
Observed Molecular Weight 76 kDa
GenBank Accession NumberBC016618
Gene Symbol SLP76
Gene ID (NCBI) 3937
RRIDAB_2881833
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LCP2, also named as SLP76, is an adapter protein that acts as a substrate of the T cell antigen receptor (TCR)-activated protein tyrosine kinase pathway. It contains an N-terminal leucine Zip motif, a tyrosine-rich domain, a proline-rich domain and a C-terminal SH2 domain. It is constitutively associated with the growth factor receptor-bound protein 2 (Grb2)-related adapter protein 2 (Grap2, a.k.a. GADS) through the interaction of its proline residues with one of the SH3 domains of Grap2. SLP76 associates with growth factor receptor bound protein 2 (Grap2), and is thought to play a role TCR-mediated intracellular signal transduction. SLP76 is also expressed by mast cells, and mast cell activation was markedly impaired in SLP76-deficient mice.

Protocols

Product Specific Protocols
WB protocol for SLP76 antibody 66465-1-IgDownload protocol
IF protocol for SLP76 antibody 66465-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols