SLC3A1 Recombinant antibody, PBS Only (Capture)

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  WB analysis of rat kidney using 84856-2-RR (same clone as 84856-2-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 84856-2-RR (SLC3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84856-2-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 84856-2-RR (same clone as 84856-2-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 84856-2-RR (SLC3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84856-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01622-2

  Cytometric bead array standard curve of MP01622-2, SLC3A1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84856-2-PBS. Detection antibody: 84856-3-PBS. Standard: Ag9525. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84856-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84856-2-RR against Human SLC3A1 were performed. The affinity constant is below 1 pM.

SLC3A1 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 84856-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

D2H, CSNU1, b(0,+)-type amino acid transporter-related heavy chain, ATR1, Amino acid transporter heavy chain SLC3A1

Formulation:  PBS Only

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Size/Concentration: 

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Freight/Packing: -

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Product Information

84856-2-PBS targets SLC3A1 as part of a matched antibody pair:

MP01622-2: 84856-2-PBS capture and 84856-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SLC3A1 fusion protein Ag9525
• Full Name: solute carrier family 3 (cystine, dibasic and neutral amino acid transporters, activator of cystine, dibasic and neutral amino acid transport), member 1
• Calculated Molecular Weight: 685 aa, 79 kDa
• Observed Molecular Weight: 78-90 kDa
• GenBank Accession Number: BC022386
• Gene Symbol: SLC3A1
• Gene ID (NCBI): 6519
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q07837
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SLC3A1(Neutral and basic amino acid transport protein rBAT) is also named as NBAT,D2h,RBAT. It is involved in the high-affinity, sodium-independent transport of cystine and neutral and dibasic amino acids (system B(0,+)-like activity).SLC3A1 can be degraded, at least in part, via the endoplasmic reticulum-associated degradation (ERAD) pathway(PMID:18332091). SLC3A1 has different isoforms vary from 79 kDa to 36kDa and the 85 kDa glycosylated protein can be detected in SDS-PAGE. (PMID:11546643)