SLC3A1 Recombinant antibody

SLC3A1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242370D3

Cat no : 84856-2-RR

Synonyms

D2H, CSNU1, b(0,+)-type amino acid transporter-related heavy chain, ATR1, Amino acid transporter heavy chain SLC3A1

---

Validation Data Gallery

• 

  WB analysis of rat kidney using 84856-2-RR

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 84856-2-RR (SLC3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 84856-2-RR

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 84856-2-RR (SLC3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84856-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84856-2-RR against Human SLC3A1 were performed. The affinity constant is below 1 pM.

---

Tested Applications

• Positive WB detected in: rat kidney tissue, mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84856-2-RR targets SLC3A1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SLC3A1 fusion protein Ag9525
• Full Name: solute carrier family 3 (cystine, dibasic and neutral amino acid transporters, activator of cystine, dibasic and neutral amino acid transport), member 1
• Calculated Molecular Weight: 685 aa, 79 kDa
• Observed Molecular Weight: 78-90 kDa
• GenBank Accession Number: BC022386
• Gene Symbol: SLC3A1
• Gene ID (NCBI): 6519
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC3A1(Neutral and basic amino acid transport protein rBAT) is also named as NBAT,D2h,RBAT. It is involved in the high-affinity, sodium-independent transport of cystine and neutral and dibasic amino acids (system B(0,+)-like activity).SLC3A1 can be degraded, at least in part, via the endoplasmic reticulum-associated degradation (ERAD) pathway(PMID:18332091). SLC3A1 has different isoforms vary from 79 kDa to 36kDa and the 85 kDa glycosylated protein can be detected in SDS-PAGE. (PMID:11546643)