SHMT1 Monoclonal antibody, PBS Only

SHMT1 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D5E6

Cat no : 67963-1-PBS

Synonyms

CSHMT, Serine methylase, SHMT, SHMT1

---

Validation Data Gallery

• 

  WB analysis of rabbit liver using 67963-1-Ig (same clone as 67963-1-PBS)

  rabbit liver tissue were subjected to SDS PAGE followed by western blot with 67963-1-Ig (SHMT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67963-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67963-1-Ig (same clone as 67963-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67963-1-Ig (SHMT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67963-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67963-1-Ig (same clone as 67963-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SHMT1 antibody (67963-1-Ig, Clone: 2D5E6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67963-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67963-1-PBS targets SHMT1 in WB, IF, Indirect ELISA applications and shows reactivity with Human, pig, rabbit samples.

• Tested Reactivity: Human, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SHMT1 fusion protein Ag5833
• Full Name: serine hydroxymethyltransferase 1 (soluble)
• Calculated Molecular Weight: 483 aa, 53 kDa
• Observed Molecular Weight: 45-53 kDa
• GenBank Accession Number: BC038598
• Gene Symbol: SHMT1
• Gene ID (NCBI): 6470
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.