SHMT1 Monoclonal antibody

SHMT1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D5E6

Cat no : 67963-1-Ig

Synonyms

CSHMT, Serine methylase, SHMT, SHMT1

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Validation Data Gallery

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  WB analysis of rabbit liver using 67963-1-Ig

  rabbit liver tissue were subjected to SDS PAGE followed by western blot with 67963-1-Ig (SHMT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67963-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67963-1-Ig (SHMT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 67963-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SHMT1 antibody (67963-1-Ig, Clone: 2D5E6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, rabbit liver tissue, HepG2 cells, Jurkat cells, K-562 cells, Pig liver tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67963-1-Ig targets SHMT1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig, rabbit samples.

• Tested Reactivity: Human, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SHMT1 fusion protein Ag5833
• Full Name: serine hydroxymethyltransferase 1 (soluble)
• Calculated Molecular Weight: 483 aa, 53 kDa
• Observed Molecular Weight: 45-53 kDa
• GenBank Accession Number: BC038598
• Gene Symbol: SHMT1
• Gene ID (NCBI): 6470
• RRID: AB_2918714
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.