SGK3 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SGK3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12699-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 12699-1-AP (same clone as 12699-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skin using 12699-1-AP (same clone as 12699-1-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 12699-1-AP (same clone as 12699-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 12699-1-AP (same clone as 12699-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat kidney using 12699-1-AP (same clone as 12699-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse heart using 12699-1-AP (same clone as 12699-1-PBS)

  IP result of anti-SGK3 (IP:12699-1-AP, 4ug; Detection:12699-1-AP 1:500) with mouse heart tissue lysate 3200ug. This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 12699-1-AP (same clone as 12699-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12699-1-AP (SGK3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 12699-1-AP (same clone as 12699-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12699-1-AP (SGK3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 12699-1-AP (same clone as 12699-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12699-1-AP (SGK3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A375 using 12699-1-AP (same clone as 12699-1-PBS)

  Immunofluorescent analysis of A375 cells using 12699-1-AP (SGK3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

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Product Information

12699-1-PBS targets SGK3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SGK3 fusion protein Ag3149
• Full Name: serum/glucocorticoid regulated kinase family, member 3
• Calculated Molecular Weight: 496 aa, 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC015326
• Gene Symbol: SGK3
• Gene ID (NCBI): 23678
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Serum- and glucocorticoid-inducible kinase 3 (SGK3) is a protein kinase of the AGC family of protein kinase A, protein kinase G, and protein kinase C and functions downstream of phosphatidylinositol 3-kinase (PI3K)(PMID:21084382). It is also named as DKFZp781N0293, CISK, SGK2, SGKL and may be involved in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. SGK3 mediates cell IL-3-dependent survival signals (PMID:12397388). SGK3 has 2 isoforms produced by alternative splicing with the molecular weight of 57 kDa and 53 kDa.