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  • Featured Product
  • KD/KO Validated

SGK3 Polyclonal antibody

SGK3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12699-1-AP

Synonyms

SGKL, SGK 3, Serum/glucocorticoid regulated kinase-like isoform 1 variant, CISK

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SGK3 Polyclonal antibody (12699-1-AP)

    WB analysis of HeLa using 12699-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using SGK3 Polyclonal antibody (12699-1-AP)

    WB analysis of mouse skin using 12699-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using SGK3 Polyclonal antibody (12699-1-AP)

    WB analysis of human liver using 12699-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using SGK3 Polyclonal antibody (12699-1-AP)

    WB analysis of A375 using 12699-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using SGK3 Polyclonal antibody (12699-1-AP)

    WB analysis of rat kidney using 12699-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using SGK3 Polyclonal antibody (12699-1-AP)

    IP experiment of mouse heart using 12699-1-AP

    IP result of anti-SGK3 (IP:12699-1-AP, 4ug; Detection:12699-1-AP 1:500) with mouse heart tissue lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SGK3 Polyclonal antibody (12699-1-AP)

    IHC staining of human pancreas cancer using 12699-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12699-1-AP (SGK3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SGK3 Polyclonal antibody (12699-1-AP)

    IHC staining of human cervical cancer using 12699-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12699-1-AP (SGK3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SGK3 Polyclonal antibody (12699-1-AP)

    IHC staining of human cervical cancer using 12699-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12699-1-AP (SGK3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using SGK3 Polyclonal antibody (12699-1-AP)

    IF Staining of A375 using 12699-1-AP

    Immunofluorescent analysis of A375 cells using 12699-1-AP (SGK3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, A375 cells, human liver tissue, mouse skin tissue, rat kidney tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman pancreas cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12699-1-AP targets SGK3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SGK3 fusion protein Ag3149
Full Name serum/glucocorticoid regulated kinase family, member 3
Calculated Molecular Weight 496 aa, 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC015326
Gene Symbol SGK3
Gene ID (NCBI) 23678
RRIDAB_2188548
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serum- and glucocorticoid-inducible kinase 3 (SGK3) is a protein kinase of the AGC family of protein kinase A, protein kinase G, and protein kinase C and functions downstream of phosphatidylinositol 3-kinase (PI3K)(PMID:21084382). It is also named as DKFZp781N0293, CISK, SGK2, SGKL and may be involved in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. SGK3 mediates cell IL-3-dependent survival signals (PMID:12397388). SGK3 has 2 isoforms produced by alternative splicing with the molecular weight of 57 kDa and 53 kDa.

Protocols

Product Specific Protocols
WB protocol for SGK3 antibody 12699-1-APDownload protocol
IHC protocol for SGK3 antibody 12699-1-APDownload protocol
IF protocol for SGK3 antibody 12699-1-APDownload protocol
IP protocol for SGK3 antibody 12699-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Autophagy

Autophagic death of neural stem cells mediates chronic stress-induced decline of adult hippocampal neurogenesis and cognitive deficits.

Authors - Seonghee Jung
  • KO Validated
View Article
mouseIF

Front Cell Dev Biol

Single-Cell RNA Sequencing With Combined Use of Bulk RNA Sequencing to Reveal Cell Heterogeneity and Molecular Changes at Acute Stage of Ischemic Stroke in Mouse Cortex Penumbra Area.

Authors - Kang Guo
View Article
mouse,ratWB,IHC,IF

Front Pharmacol

The role of the SGK3/TOPK signaling pathway in the transition from acute kidney injury to chronic kidney disease

Authors - Huapan Shu
  • KD Validated
View Article