HeLa cells were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse skin using 12699-1-AP
mouse skin tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
mouse skin tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 12699-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
human liver tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 12699-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
A375 cells were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 12699-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
rat kidney tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 12699-1-AP
IP result of anti-SGK3 (IP:12699-1-AP, 4ug; Detection:12699-1-AP 1:500) with mouse heart tissue lysate 3200ug.
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12699-1-AP (SGK3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human cervical cancer using 12699-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12699-1-AP (SGK3 Antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12699-1-AP (SGK3 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human cervical cancer using 12699-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12699-1-AP (SGK3 Antibody) at dilution of 1:50 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12699-1-AP (SGK3 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of A375 using 12699-1-AP
Immunofluorescent analysis of A375 cells using 12699-1-AP (SGK3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of A375 cells using 12699-1-AP (SGK3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HeLa cells, A375 cells, human liver tissue, mouse skin tissue, rat kidney tissue
Positive IP detected in
mouse heart tissue
Positive IHC detected in
human pancreas cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
A375 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Serum- and glucocorticoid-inducible kinase 3 (SGK3) is a protein kinase of the AGC family of protein kinase A, protein kinase G, and protein kinase C and functions downstream of phosphatidylinositol 3-kinase (PI3K)(PMID:21084382). It is also named as DKFZp781N0293, CISK, SGK2, SGKL and may be involved in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. SGK3 mediates cell IL-3-dependent survival signals (PMID:12397388). SGK3 has 2 isoforms produced by alternative splicing with the molecular weight of 57 kDa and 53 kDa.
Single-Cell RNA Sequencing With Combined Use of Bulk RNA Sequencing to Reveal Cell Heterogeneity and Molecular Changes at Acute Stage of Ischemic Stroke in Mouse Cortex Penumbra Area.
HeLa cells were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse skin using 12699-1-AP
mouse skin tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 12699-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 12699-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 12699-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human pancreas cancer using 12699-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12699-1-AP (SGK3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human cervical cancer using 12699-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12699-1-AP (SGK3 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human cervical cancer using 12699-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12699-1-AP (SGK3 Antibody) at dilution of 1:50 (under 40x lens).
IP Figures
IP experiment of mouse heart using 12699-1-AP
IP result of anti-SGK3 (IP:12699-1-AP, 4ug; Detection:12699-1-AP 1:500) with mouse heart tissue lysate 3200ug.
IF/ICC Figures
IF Staining of A375 using 12699-1-AP
Immunofluorescent analysis of A375 cells using 12699-1-AP (SGK3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
SGK3 Polyclonal antibody
Catalog Number
12699-1-AP
Citations
3
Dilutions
WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:10-1:100
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
