SERPING1/C1 Inactivator Monoclonal antibody

• Featured Product
• KD/KO Validated

SERPING1/C1 Inactivator Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig, rat and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1B11B11

Cat no : 66882-1-Ig

Synonyms

C1 esterase inhibitor, C1 Inh, C1 inhibiting factor, C1IN, C1INH, C1NH, HAE1, HAE2, Plasma protease C1 inhibitor, Serpin G1, SERPING1, SERPING1/C1 Inactivator

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Validation Data Gallery

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  WB analysis using 66882-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66882-1-Ig (SERPING1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 66882-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SERPING1/C1 Inactivator antibody (66882-1-Ig, Clone: 1B11B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 66882-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SERPING1/C1 Inactivator antibody (66882-1-Ig, Clone: 1B11B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66882-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SERPING1/C1 Inactivator antibody (66882-1-Ig, Clone: 1B11B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: human plasma tissue, human plasma, pig plasma, rat serum
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

66882-1-Ig targets SERPING1/C1 Inactivator in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig, rat samples.

• Tested Reactivity: Human, pig, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SERPING1/C1 Inactivator fusion protein Ag16782
• Full Name: serpin peptidase inhibitor, clade G (C1 inhibitor), member 1
• Calculated Molecular Weight: 500 aa, 55 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC011171
• Gene Symbol: SERPING1
• Gene ID (NCBI): 710
• RRID: AB_2882214
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPING1, also known as C1 Inhibitor (C1INH), is a member of the Serine proteinase inhibitor family. Its main function is the inhibition of the complement system to prevent spontaneous activation. It inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. Deficiency of this protein is associated with hereditary angioneurotic oedema (HANE). C1INH is the most heavily glycosylated plasma protein.

Publications

Species	Application	Title
human	WB	iScience; Targeting endometrial inflammation in intrauterine adhesion ameliorates endometrial fibrosis by priming MSCs to secrete C1INH; Authors - Simin Yao ; KD Validated