Alpha Antichymotrypsin Monoclonal antibody, PBS Only

Alpha Antichymotrypsin Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F2F10

Cat no : 66078-1-PBS

Synonyms

A1 Antichymotrypsin, A-1-Antichymotrypsin, AACT, ACT, Alpha 1 antichymotrypsin, Alpha Antichymotrypsin, GIG24, GIG25, Serpin A3, SERPINA3

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Validation Data Gallery

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  WB analysis of human plasma using 66078-1-Ig (same clone as 66078-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66078-1-Ig (Alpha Antichymotrypsin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human plasma using 66078-1-Ig (same clone as 66078-1-PBS)

  Untreated and PNGase F-treated lysates of human plasma were subjected to SDS PAGE followed by western blot with 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. Loading amount:0.7 μL crude plasma was lysised for each lane. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human colon using 66078-1-Ig (same clone as 66078-1-PBS)

  Untreated and PNGase F-treated lysates of human colon tissue (25μg/lane) were subjected to SDS PAGE followed by western blot with 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66078-1-Ig (same clone as 66078-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66078-1-Ig (same clone as 66078-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66078-1-Ig (same clone as 66078-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66078-1-Ig(alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66078-1-Ig (same clone as 66078-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66078-1-Ig(alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66078-1-Ig (same clone as 66078-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

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Product Information

66078-1-PBS targets Alpha Antichymotrypsin in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Alpha Antichymotrypsin fusion protein Ag2830
• Full Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
• Calculated Molecular Weight: 448 aa, 48 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC010530
• Gene Symbol: Alpha 1 Antichymotrypsin
• Gene ID (NCBI): 12
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Human SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a member of the serine protease inhibitor class, and is a plasma alpha globulin glycoprotein which increases in the blood during the inflammatory process. SerpinA3, is also an inhibitor of neutrophil cathepsin G, mast cell chymases and pancreatic chymotrypsin. SerpinA3 is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease. SerpinA3 has also been implicated in the pathology of a number of devastating human diseases including chronic obstructive pulmonary disease (COPD), Parkinson's disease (PD), Alzheimer's disease (AD), Stroke, Cystic Fibrosis, Cerebral Haemorrhage and Multiple System Atrophy.