SERCA3 Monoclonal antibody, PBS Only

SERCA3 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A2C2

Cat no : 68287-1-PBS

Synonyms

ATP2A3, Calcium pump 3, SERCA3, SR Ca(2+) ATPase 3

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Validation Data Gallery

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  WB analysis using 68287-1-Ig (same clone as 68287-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68287-1-Ig (ATP2A3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68287-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68287-1-Ig (same clone as 68287-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68287-1-Ig (ATP2A3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68287-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HT-29 using 68287-1-Ig (same clone as 68287-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using SERCA3 antibody (68287-1-Ig, Clone: 1A2C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68287-1-PBS in a different storage buffer formulation.

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Product Information

68287-1-PBS targets SERCA3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SERCA3 fusion protein Ag4527
• Full Name: ATPase, Ca++ transporting, ubiquitous
• Calculated Molecular Weight: 1043 aa, 114 kDa
• Observed Molecular Weight: 97-110 kDa
• GenBank Accession Number: BC035729
• Gene Symbol: SERCA3
• Gene ID (NCBI): 489
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The SERCA, which pumps cytosolic Ca2+ into the ER, is an imperative maintainer of ER Ca2+ homeostasis. The mammalian SERCA family is comprised of three tissue-specific members, SERCA1-3. SERCA3 is present in endothelial and epithelial cells of various tissues. SERCA3 helps regulate intracellular Ca2+, which is critical for maintaining normal INS secretion and is closely linked to metabolism, neuronal plasticity, gene transcription, cell growth, differentiation, apoptosis, protein folding and carcinogenesis. Several isoforms of SERCA3 exist due to the alternative splicing.