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SERCA3 Monoclonal antibody

SERCA3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A2C2

Cat no : 68287-1-Ig

Synonyms

ATP2A3, Calcium pump 3, SERCA3, SR Ca(2+) ATPase 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SERCA3 Monoclonal antibody (68287-1-Ig)

    WB analysis using 68287-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68287-1-Ig (ATP2A3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • Western Blot (WB) analysis of various lysates using SERCA3 Monoclonal antibody (68287-1-Ig)

    WB analysis using 68287-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68287-1-Ig (ATP2A3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using SERCA3 Monoclonal antibody (68287-1-Ig)

    IF Staining of HT-29 using 68287-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using SERCA3 antibody (68287-1-Ig, Clone: 1A2C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, mouse heart tissue, MOLT-4 cells, Jurkat cells, K-562 cells, HL-60 cells, rat heart tissue
Positive IF/ICC detected inHT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68287-1-Ig targets SERCA3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SERCA3 fusion protein Ag4527
Full Name ATPase, Ca++ transporting, ubiquitous
Calculated Molecular Weight 1043 aa, 114 kDa
Observed Molecular Weight 97-110 kDa
GenBank Accession NumberBC035729
Gene Symbol SERCA3
Gene ID (NCBI) 489
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The SERCA, which pumps cytosolic Ca2+ into the ER, is an imperative maintainer of ER Ca2+ homeostasis. The mammalian SERCA family is comprised of three tissue-specific members, SERCA1-3. SERCA3 is present in endothelial and epithelial cells of various tissues. SERCA3 helps regulate intracellular Ca2+, which is critical for maintaining normal INS secretion and is closely linked to metabolism, neuronal plasticity, gene transcription, cell growth, differentiation, apoptosis, protein folding and carcinogenesis. Several isoforms of SERCA3 exist due to the alternative splicing.

Protocols

Product Specific Protocols
WB protocol for SERCA3 antibody 68287-1-IgDownload protocol
IF protocol for SERCA3 antibody 68287-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols