CoraLite® Plus 488-conjugated SEPT6 Monoclonal antibody

SEPT6 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rabbit, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B1G1

Cat no : CL488-68173

Synonyms

KIAA0128, SEP2, SEPT2, SEPT6, septin 6

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Validation Data Gallery

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  IF Staining of HEK-293 using CL488-68173

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 SEPT6 antibody (CL488-68173, Clone: 1B1G1 ) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68173 targets SEPT6 in IF/ICC applications and shows reactivity with Human, mouse, pig, rabbit, rat samples.

• Tested Reactivity: Human, mouse, pig, rabbit, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SEPT6 fusion protein Ag32736
• Full Name: septin 6
• Calculated Molecular Weight: 434 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC036240
• Gene Symbol: SEPT6
• Gene ID (NCBI): 23157
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Septin 6 belongs to the septins family which are conserved GTP-binding proteins functioning as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeletal remodeling. Septin 6 also interacts with hepatitis C virus (HCV) NS5b RNA polymerase and NS5b-binding protein ( HNRNPA1) and may play a role in HCV RNA replication.