Various lysates were subjected to SDS PAGE followed by western blot with 68173-1-Ig (SEPT6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 68173-1-Ig (SEPT6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
IF Staining of HEK-293 using 68173-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SEPT6 antibody (68173-1-Ig, Clone: 1B1G1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Septin 6 belongs to the septins family which are conserved GTP-binding proteins functioning as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeletal remodeling. Septin 6 also interacts with hepatitis C virus (HCV) NS5b RNA polymerase and NS5b-binding protein ( HNRNPA1) and may play a role in HCV RNA replication.
Various lysates were subjected to SDS PAGE followed by western blot with 68173-1-Ig (SEPT6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
IF/ICC Figures
IF Staining of HEK-293 using 68173-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SEPT6 antibody (68173-1-Ig, Clone: 1B1G1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
SEPT6 Monoclonal antibody
Catalog Number
68173-1-Ig
Citations
-
Dilutions
WB : 1:5000-1:50000 IF/ICC : 1:400-1:1600
Applications
WB, IF/ICC, ELISA
Reactivity
human, mouse, pig, rabbit, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet