SEPT6 Monoclonal antibody

SEPT6 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rabbit, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B1G1

Cat no : 68173-1-Ig

Synonyms

KIAA0128, SEP2, SEPT2, SEPT6, septin 6

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Validation Data Gallery

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  WB analysis using 68173-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68173-1-Ig (SEPT6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IF Staining of HEK-293 using 68173-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SEPT6 antibody (68173-1-Ig, Clone: 1B1G1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, Pig brain tissue, Rabbit brain tissue, Rat brain tissue, Mouse brain tissue
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68173-1-Ig targets SEPT6 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, pig, rabbit, rat samples.

• Tested Reactivity: Human, mouse, pig, rabbit, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SEPT6 fusion protein Ag32736
• Full Name: septin 6
• Calculated Molecular Weight: 434 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC036240
• Gene Symbol: SEPT6
• Gene ID (NCBI): 23157
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Septin 6 belongs to the septins family which are conserved GTP-binding proteins functioning as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeletal remodeling. Septin 6 also interacts with hepatitis C virus (HCV) NS5b RNA polymerase and NS5b-binding protein ( HNRNPA1) and may play a role in HCV RNA replication.