Anti-Human SEMA7A Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98324-3-RR

  1x10^6 untreated or PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human SEMA7A Rabbit RecAb (98324-3-RR, Clone: 241814B12) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of human PBMCs using 98324-3-RR

  1x10^6 PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human SEMA7A Rabbit RecAb (98324-3-RR, Clone: 241814B12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human SEMA7A Rabbit RecAb (98324-3-RR, Clone: 241814B12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed.

SEMA7A Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98324-3-RR

Clone No.241814B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD108, CDw108, John-Milton-Hargen human blood group Ag, Sema K1, Sema L

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Tested Applications

• Positive FC detected in: PHA treated human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98324-3-RR targets SEMA7A in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SEMA7A fusion protein Eg2005
• Full Name: semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group)
• Calculated Molecular Weight: 75kDa
• GenBank Accession Number: NM_003612.5
• Gene Symbol: SEMA7A
• Gene ID (NCBI): 8482
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75326-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

SEMA7A, also named CD108 and SEMAL, belongs to the semaphorin family. SEMA7A is the only membrane-associated glycosylphosphatidylinositol (GPI)-linked semaphorin and plays a role in the regulation of neuronal axon outgrowth. In addition to its role in guiding axon pathfinding during neuronal development, SEMA7A has diverse functions in morphogenesis and immune cell control and regulates T cell responses via the α1β1 integrin receptor.