SEMA3A Recombinant antibody, PBS Only

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  WB analysis using 83579-5-RR (same clone as 83579-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83579-5-RR (SEMA3A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83579-5-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83579-5-RR (same clone as 83579-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83579-5-RR (SEMA3A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83579-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 83579-5-RR (same clone as 83579-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83579-5-RR (SEMA3A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83579-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83579-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83579-5-RR against Human SEMA3A were performed. The affinity constant is 0.228 nM.

SEMA3A {ptg:Brand} Recombinant Antibody for WB, IHC, ELISA

Cat No. 83579-5-PBS

Clone No.240509E3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

SEMA1, Sema III, Hsema III, Hsema I, coll 1

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Product Information

83579-5-PBS targets SEMA3A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SEMA3A fusion protein Ag27173
• Full Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
• Calculated Molecular Weight: 771 aa, 89 kDa
• Observed Molecular Weight: 89 kDa
• GenBank Accession Number: BC111416
• Gene Symbol: SEMA3A
• Gene ID (NCBI): 10371
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q14563
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

SEMA3A (Semaphorin 3A), a class-3 semaphorin signaling protein, is involved in cell signaling with PlexinA1 and Neuropilin-1 (NRP1) receptors and it is responsible for recruiting dendritic cells into lymphatics. It's widely expressed in different tissues (PMID:10766232). SEMA3A has been shown to exert osteoprotective effects, characterized by increasing bone formation while inhibiting the activation of osteoclast precursor cells (PMID:36598593). However, SEMA3A also modulates the immune system. SEMA3A expression in a myocardial infarct area enhances macrophage polarization and inhibits monocyte migration into the lesion area (PMID:28540528).