SEMA3A Recombinant antibody

SEMA3A Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240509E3

Cat no : 83579-5-RR

Synonyms

SEMA1, Sema III, Hsema III, Hsema I, coll 1

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Validation Data Gallery

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  WB analysis using 83579-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83579-5-RR (SEMA3A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83579-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83579-5-RR against Human SEMA3A were performed. The affinity constant is 0.228 nM.

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Tested Applications

• Positive WB detected in: SKOV-3 cells, PC-12 cells, C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83579-5-RR is SEMA3A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SEMA3A fusion protein Ag27173
• Full Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
• Calculated Molecular Weight: 771 aa, 89 kDa
• Observed Molecular Weight: 89 kDa
• GenBank Accession Number: BC111416
• Gene Symbol: SEMA3A
• Gene ID (NCBI): 10371
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEMA3A (Semaphorin 3A), a class-3 semaphorin signaling protein, is involved in cell signaling with PlexinA1 and Neuropilin-1 (NRP1) receptors and it is responsible for recruiting dendritic cells into lymphatics. It's widely expressed in different tissues (PMID:10766232). SEMA3A has been shown to exert osteoprotective effects, characterized by increasing bone formation while inhibiting the activation of osteoclast precursor cells (PMID:36598593). However, SEMA3A also modulates the immune system. SEMA3A expression in a myocardial infarct area enhances macrophage polarization and inhibits monocyte migration into the lesion area (PMID:28540528).