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SEMA3A Recombinant antibody

SEMA3A Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240509E3

Cat no : 83579-5-RR

Synonyms

SEMA1, Sema III, Hsema III, Hsema I, coll 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEMA3A Recombinant antibody (83579-5-RR)

    WB analysis using 83579-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83579-5-RR (SEMA3A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83579-5-RR

    Affinity and Kinetic Characterization of 83579-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83579-5-RR against Human SEMA3A were performed. The affinity constant is 0.228 nM.

Tested Applications

Positive WB detected inSKOV-3 cells, PC-12 cells, C6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83579-5-RR is SEMA3A Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SEMA3A fusion protein Ag27173
Full Name sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
Calculated Molecular Weight 771 aa, 89 kDa
Observed Molecular Weight89 kDa
GenBank Accession NumberBC111416
Gene Symbol SEMA3A
Gene ID (NCBI) 10371
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEMA3A (Semaphorin 3A), a class-3 semaphorin signaling protein, is involved in cell signaling with PlexinA1 and Neuropilin-1 (NRP1) receptors and it is responsible for recruiting dendritic cells into lymphatics. It's widely expressed in different tissues (PMID:10766232). SEMA3A has been shown to exert osteoprotective effects, characterized by increasing bone formation while inhibiting the activation of osteoclast precursor cells (PMID:36598593). However, SEMA3A also modulates the immune system. SEMA3A expression in a myocardial infarct area enhances macrophage polarization and inhibits monocyte migration into the lesion area (PMID:28540528).

Protocols

Product Specific Protocols
WB protocol for SEMA3A antibody 83579-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols