CoraLite® Plus 488-conjugated SEC63 Monoclonal antibody

SEC63 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C10E1

Cat no : CL488-67352

Synonyms

ERdj2, PRO2507, SEC63, SEC63 homolog (S. cerevisiae), SEC63L

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Validation Data Gallery

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  IF Staining of HEK-293 using CL488-67352

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 SEC63 antibody (CL488-67352, Clone: 1C10E1 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67352 targets SEC63 in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SEC63 fusion protein Ag5783
• Full Name: SEC63 homolog (S. cerevisiae)
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC047221
• Gene Symbol: SEC63
• Gene ID (NCBI): 11231
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SEC63 is a integral ER membrane protein. SEC63 and SEC62 form a dimeric complex and play a central role in translocation of nascent and newly synthesized precursor polypeptides into the ER. Mutations in the gene encoding SEC63 cause polycystic liver disease in humans. The intact SEC63 protein is usually detected around 83-90 kDa, while smaller cleavage products can be observed by autophagy proteolysis (PMID: 25052096)