SEC13 Recombinant antibody

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  WB analysis using 85110-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85110-4-RR (SEC13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 85110-4-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SEC13 antibody (85110-4-RR, Clone: 242775C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 85110-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85110-4-RR against Human SEC13 were performed. The affinity constant is 0.114 nM.

SEC13 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85110-4-RR

Clone No.242775C3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

SEC13L1, SEC13A, Protein SEC13 homolog, GATOR2 complex protein SEC13, D3S1231E

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Tested Applications

• Positive WB detected in: LNCaP cells, HEK-293 cells, HepG2 cells, A431 cells, RAW 264.7 cells, mouse pancreas tissue, rat pancreas tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85110-4-RR targets SEC13 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SEC13 fusion protein Ag7610
• Full Name: SEC13 homolog (S. cerevisiae)
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC002634
• Gene Symbol: SEC13
• Gene ID (NCBI): 6396
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P55735
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEC13 is a component of the nuclear pore complex (NPC) and the COPII coat. It is required for esicle biogenesis from endoplasmic reticulum during the transport of proteins. Shuttling of SEC13 between the nucleus and the cytoplasm may couple and regulate functions between these two compartments. (PMID: 14517296; 16407955)