SEC13 Recombinant antibody, PBS Only

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  WB analysis using 85110-4-RR (same clone as 85110-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85110-4-RR (SEC13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85110-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 85110-4-RR (same clone as 85110-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SEC13 antibody (85110-4-RR, Clone: 242775C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85110-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85110-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85110-4-RR against Human SEC13 were performed. The affinity constant is 0.114 nM.

SEC13 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 85110-4-PBS

Clone No.242775C3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

SEC13L1, SEC13A, Protein SEC13 homolog, GATOR2 complex protein SEC13, D3S1231E

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Product Information

85110-4-PBS targets SEC13 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SEC13 fusion protein Ag7610
• Full Name: SEC13 homolog (S. cerevisiae)
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC002634
• Gene Symbol: SEC13
• Gene ID (NCBI): 6396
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P55735
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

SEC13 is a component of the nuclear pore complex (NPC) and the COPII coat. It is required for esicle biogenesis from endoplasmic reticulum during the transport of proteins. Shuttling of SEC13 between the nucleus and the cytoplasm may couple and regulate functions between these two compartments. (PMID: 14517296; 16407955)