CoraLite® Plus 488-conjugated SDHA Monoclonal antibody

SDHA Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5E10G12

Cat no : CL488-66588

Synonyms

PPGL5, PGL5, MC2DN1, FP, EC:1.3.5.1

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-66588

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488-conjugated SDHA antibody (CL488-66588, Clone: 5E10G12 ) at dilution of 1:100. Red: CL594-Phalloidin stains F-Actin; Blue: DAPI.

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  IF Staining of HepG2 using CL488-66588

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66588 (SDHA antibody) at dilution of 1:100.

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  FC experiment of HeLa using CL488-66588

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SDHA (CL488-66588, Clone:5E10G12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66588 targets SDHA in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SDHA fusion protein Ag7023
• Full Name: succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 62-73 kDa
• GenBank Accession Number: BC001380
• Gene Symbol: SDHA
• Gene ID (NCBI): 6389
• RRID: AB_2883359
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) can be removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor.