SDHA Monoclonal antibody

SDHA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, rabbit, pig and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

5E10G12

Reviews (1)

Cat no : 66588-1-Ig

Synonyms

FP, SDH2, SDHA, SDHF

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Validation Data Gallery

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  WB analysis using 66588-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66588-1-Ig (SDHA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66588-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66588-1-Ig (SDHA antibody) at dilution of 1:2000 (under 10x lens).

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  IHC staining of human liver cancer using 66588-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66588-1-Ig (SDHA antibody) at dilution of 1:2000 (under 40x lens).

• 

  IF Staining of HepG2 using 66588-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66588-1-Ig (SDHA antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, pig brain tissue, rat brain tissue, mouse brain tissue, rabbit brain tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

66588-1-Ig targets SDHA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, rabbit, pig samples.

• Tested Reactivity: Human, Mouse, Rat, rabbit, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SDHA fusion protein Ag7023
• Full Name: succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 62-73 kDa
• GenBank Accession Number: BC001380
• Gene Symbol: SDHA
• Gene ID (NCBI): 6389
• RRID: AB_2881948
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) can be removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor.

Publications

Species	Application	Title
human	WB	Nat Commun; Selective activator of human ClpP triggers cell cycle arrest to inhibit lung squamous cell carcinoma; Authors - Lin-Lin Zhou
human	WB	Cell Rep; Cardiolipin oxidized by ROS from complex II acts as a target of gasdermin D to drive mitochondrial pore and heart dysfunction in endotoxemia; Authors - Yan Tang
		Sci China Life Sci; Cholesterol transport through the peroxisome-ER membrane contacts tethered by PI(4,5)P2 and extended synaptotagmins.; Authors - Jian Xiao
human,mouse	WB	Cell Chem Biol; Aberrant human ClpP activation disturbs mitochondrial proteome homeostasis to suppress pancreatic ductal adenocarcinoma.; Authors - Pengyu Wang
human	WB	Bioorg Chem; SAR-guided development of indole-matrine hybrids as potential anticancer agents via mitochondrial stress/cytochrome c/caspase 3 signaling pathway; Authors - Lingyu Li
mouse	WB	bioRxiv; FAM210A Regulates Mitochondrial Translation and Maintains Cardiac Mitochondrial Homeostasis; Authors - Jiangbin Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (07-12-2023)

Great antibody for WB. Very strong signal and clean background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: Mouse liver

SDHA Antibody Western Blot validation (1:10000 dilution) in Mouse liver (Cat no:66588-1-Ig)