SDHA Monoclonal antibody, PBS Only (Capture)

SDHA Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5E10G12

Cat no : 66588-1-PBS

Synonyms

PPGL5, PGL5, MC2DN1, FP, EC:1.3.5.1

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Validation Data Gallery

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  WB analysis using 66588-1-Ig (same clone as 66588-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66588-1-Ig (SDHA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66588-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66588-1-Ig (same clone as 66588-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66588-1-Ig (SDHA antibody) at dilution of 1:2000 (under 10x lens). This data was developed using the same antibody clone with 66588-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66588-1-Ig (same clone as 66588-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66588-1-Ig (SDHA antibody) at dilution of 1:2000 (under 40x lens). This data was developed using the same antibody clone with 66588-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66588-1-Ig (same clone as 66588-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66588-1-Ig (SDHA antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66588-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50790-1

  Cytometric bead array standard curve of MP50790-1, SDHA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66588-1-PBS. Detection antibody: 66588-2-PBS. Standard:Ag7023. Range: 0.781-200 ng/mL.

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  Cytometric bead array standard curve of MP50790-2

  Cytometric bead array standard curve of MP50790-2, SDHA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66588-1-PBS. Detection antibody: 66588-3-PBS. Standard:Ag7023. Range: 0.781-200 ng/mL.

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  Cytometric bead array standard curve of MP50790-3

  Cytometric bead array standard curve of MP50790-3, SDHA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66588-1-PBS. Detection antibody: 66588-4-PBS. Standard:null. Range: null.

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Product Information

66588-1-PBS targets SDHA as part of a matched antibody pair:

MP50790-1: 66588-1-PBS capture and 66588-2-PBS detection (validated in Cytometric bead array)

MP50790-2: 66588-1-PBS capture and 66588-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SDHA fusion protein Ag7023
• Full Name: succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 62-73 kDa
• GenBank Accession Number: BC001380
• Gene Symbol: SDHA
• Gene ID (NCBI): 6389
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) can be removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor.