SDHA Monoclonal antibody
SDHA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, rabbit, pig and More (2)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
5E10G12
Cat no : 66588-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells, pig brain tissue, rat brain tissue, mouse brain tissue, rabbit brain tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
Product Information
66588-1-Ig targets SDHA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, rabbit, pig samples.
| Tested Reactivity | Human, Mouse, Rat, rabbit, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SDHA fusion protein Ag7023 |
| Full Name | succinate dehydrogenase complex, subunit A, flavoprotein (Fp) |
| Calculated Molecular Weight | 73 kDa |
| Observed Molecular Weight | 62-73 kDa |
| GenBank Accession Number | BC001380 |
| Gene Symbol | SDHA |
| Gene ID (NCBI) | 6389 |
| RRID | AB_2881948 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) can be removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SDHA antibody 66588-1-Ig | Download protocol |
| IHC protocol for SDHA antibody 66588-1-Ig | Download protocol |
| IF protocol for SDHA antibody 66588-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Selective activator of human ClpP triggers cell cycle arrest to inhibit lung squamous cell carcinoma | ||
Cell Rep Cardiolipin oxidized by ROS from complex II acts as a target of gasdermin D to drive mitochondrial pore and heart dysfunction in endotoxemia | ||
Sci China Life Sci Cholesterol transport through the peroxisome-ER membrane contacts tethered by PI(4,5)P2 and extended synaptotagmins. | ||
Cell Chem Biol Aberrant human ClpP activation disturbs mitochondrial proteome homeostasis to suppress pancreatic ductal adenocarcinoma. | ||
Bioorg Chem SAR-guided development of indole-matrine hybrids as potential anticancer agents via mitochondrial stress/cytochrome c/caspase 3 signaling pathway | ||
bioRxiv FAM210A Regulates Mitochondrial Translation and Maintains Cardiac Mitochondrial Homeostasis |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Hua (Verified Customer) (07-12-2023) | Great antibody for WB. Very strong signal and clean background.
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