CoraLite®594-conjugated SDCCAG8 Polyclonal antibody

• Featured Product
• KD/KO Validated

SDCCAG8 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish, Canine

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-13471

Synonyms

Antigen NY CO 8, BBS16, CCCAP, hCCCAP, HSPC085, NY CO 8, SDCCAG8

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Validation Data Gallery

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  IF Staining of MDCK using CL594-13471

  Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CoraLite®594 SDCCAG8 antibody (CL594-13471) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: MDCK cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-13471 targets SDCCAG8 in IF/ICC applications and shows reactivity with human, mouse, rat, zebrafish, Canine samples.

• Tested Reactivity: human, mouse, rat, zebrafish, Canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SDCCAG8 fusion protein Ag4264
• Full Name: serologically defined colon cancer antigen 8
• Calculated Molecular Weight: 713 aa, 83 kDa
• GenBank Accession Number: BC032454
• Gene Symbol: SDCCAG8
• Gene ID (NCBI): 10806
• RRID: AB_2919796
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SDCCAG8, also named as CCCAP and NY-CO-8, plays a role in the establishment of cell polarity and epithelial lumen formation. It may play a role in ciliogenesis. Loss of SDCCAG8 function as a cause of a retinal-renal ciliopathy and validates exome capture analysis for broadly heterogeneous single-gene disorders. SDCCAG8 is localized at both centrioles and interacts directly with OFD1 (oral-facial-digital syndrome 1), which is associated with NPHP-RC. SDCCAG8 has 4 isoforms with MW 83,78,73 and 41 kDa (refer to UniProt). Catalog#13471-1-AP can recognize all the four isoforms.