SDCCAG8 Polyclonal antibody

• Featured Product
• KD/KO Validated

SDCCAG8 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine, zebrafish

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 13471-1-AP

Synonyms

NPHP10, HSPC085, hCCCAP, CCCAP, BBS16

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Validation Data Gallery

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  IHC staining of human colon using 13471-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13471-1-AP (SDCCAG8 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 13471-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13471-1-AP (SDCCAG8 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 13471-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SDCCAG8 antibody (13471-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 9 publications below
• IP: See 1 publications below

Product Information

13471-1-AP targets SDCCAG8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine, zebrafish samples.

• Tested Reactivity: human, mouse, rat, canine, zebrafish
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SDCCAG8 fusion protein Ag4264
• Full Name: serologically defined colon cancer antigen 8
• Calculated Molecular Weight: 713 aa, 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC032454
• Gene Symbol: SDCCAG8
• Gene ID (NCBI): 10806
• RRID: AB_2183417
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SDCCAG8, also named as CCCAP and NY-CO-8, plays a role in the establishment of cell polarity and epithelial lumen formation. It may play a role in ciliogenesis. Loss of SDCCAG8 function as a cause of a retinal-renal ciliopathy and validates exome capture analysis for broadly heterogeneous single-gene disorders. SDCCAG8 is localized at both centrioles and interacts directly with OFD1 (oral-facial-digital syndrome 1), which is associated with NPHP-RC. SDCCAG8 has 4 isoforms with MW 83,78,73 and 41 kDa (refer to UniProt). Catalog#13471-1-AP can recognize all the four isoforms.

Publications

Species	Application	Title
human	WB	Nature; Global landscape of HIV-human protein complexes.; Authors - Stefanie Jäger
human, zebrafish	WB,IF	Nat Genet; Candidate exome capture identifies mutation of SDCCAG8 as the cause of a retinal-renal ciliopathy.; Authors - Otto Edgar A EA
mouse	WB, IF, IP	Neuron; SDCCAG8 Regulates Pericentriolar Material Recruitment and Neuronal Migration in the Developing Cortex.; Authors - Ryan Insolera
mouse	WB, IHC, IF	J Am Soc Nephrol; Renal-retinal ciliopathy gene sdccag8 regulates DNA damage response signaling.; Authors - Rannar Airik ; KD Validated
human	IF	J Cell Biol; Centrobin controls primary ciliogenesis in vertebrates.; Authors - Yetunde Adesanya Ogungbenro
mouse	WB, IF	Cell Death Dis; Selective loss of RPGRIP1-dependent ciliary targeting of NPHP4, RPGR and SDCCAG8 underlies the degeneration of photoreceptor neurons.; Authors - Patil H H