SCD Recombinant antibody

• 

  WB analysis using 81468-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81468-2-RR (SCD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HepG2 using 81468-2-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.2 ug SCD Recombinant Antibody (81468-2-RR, Clone:241487B5) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of MCF-7 using 81468-2-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.2 ug SCD Recombinant Antibody (81468-2-RR, Clone:241487B5) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

SCD Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 81468-2-RR

Clone No.241487B5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Delta-9 desaturase, Delta(9)-desaturase, Delta(9) desaturase, Acyl-CoA desaturase, Acyl CoA desaturase

---

Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, A375 cells, A549 cells, HEK-293T cells
• Positive FC (Intra) detected in: MCF-7 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81468-2-RR targets SCD in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SCD fusion protein Ag29156
• Full Name: stearoyl-CoA desaturase (delta-9-desaturase)
• Calculated Molecular Weight: 355 aa, 41 kDa
• Observed Molecular Weight: 28-42 kDa
• GenBank Accession Number: BC005807
• Gene Symbol: SCD
• Gene ID (NCBI): 6319
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00767
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCD1 (stearoyl-CoA desaturase) is a microsomal fatty acid monodesaturase, which catalyses the committed step in the biosynthesis of mono-unsaturated fatty acids from saturated fatty acids (PMID:10946019). SCD1 and SCD2 are the main isoforms expressed in mouse liver and brain respectively (PMID:15907797). The formation of homodimers and oligomers is an intrinsic property of SCD proteins. SCD1 is a multi-pass membrane protein and detected double bands of 37-42 kDa. The degradation product of 28 kDa may be caused by a major cleavage site at the C-terminus (PMID:15610069, PMID: 9843580).