SCD Recombinant antibody, PBS Only (Capture)

SCD Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241487B5

Cat no : 81468-2-PBS

Synonyms

Delta-9 desaturase, Delta(9)-desaturase, Delta(9) desaturase, Acyl-CoA desaturase, Acyl CoA desaturase

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Validation Data Gallery

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  WB analysis using 81468-2-RR (same clone as 81468-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81468-2-RR (SCD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81468-2-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 81468-2-RR (same clone as 81468-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.2 ug SCD Recombinant Antibody (81468-2-RR, Clone:241487B5) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81468-2-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 81468-2-RR (same clone as 81468-2-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.2 ug SCD Recombinant Antibody (81468-2-RR, Clone:241487B5) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81468-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01272-1

  Cytometric bead array standard curve of MP01272-1, SCD Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 81468-2-PBS. Detection antibody: 81468-3-PBS. Standard: Ag29156. Range: 0.313-40 ng/mL.

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Product Information

81468-2-PBS targets SCD as part of a matched antibody pair:

MP01272-1: 81468-2-PBS capture and 81468-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SCD fusion protein Ag29156
• Full Name: stearoyl-CoA desaturase (delta-9-desaturase)
• Calculated Molecular Weight: 355 aa, 41 kDa
• Observed Molecular Weight: 28-42 kDa
• GenBank Accession Number: BC005807
• Gene Symbol: SCD
• Gene ID (NCBI): 6319
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SCD1 (stearoyl-CoA desaturase) is a microsomal fatty acid monodesaturase, which catalyses the committed step in the biosynthesis of mono-unsaturated fatty acids from saturated fatty acids (PMID:10946019). SCD1 and SCD2 are the main isoforms expressed in mouse liver and brain respectively (PMID:15907797). The formation of homodimers and oligomers is an intrinsic property of SCD proteins. SCD1 is a multi-pass membrane protein and detected double bands of 37-42 kDa. The degradation product of 28 kDa may be caused by a major cleavage site at the C-terminus (PMID:15610069, PMID: 9843580).