SAR1A Recombinant antibody, PBS Only

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  IF Staining of HepG2 using 83168-7-RR (same clone as 83168-7-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SAR1A antibody (83168-7-RR, Clone: 230471A1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83168-7-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 83168-7-RR (same clone as 83168-7-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug SAR1A Recombinant antibody (83168-7-RR, Clone:230471A1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83168-7-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 83168-7-RR (same clone as 83168-7-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug SAR1A Recombinant antibody (83168-7-RR, Clone:230471A1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83168-7-PBS in a different storage buffer formulation.

SAR1A {ptg:Brand} Recombinant Antibody for IF/ICC, ELISA

Cat No. 83168-7-PBS

Clone No.230471A1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

SAR1, GTP binding protein SAR1a, EC:3.6.5.2, COPII-associated small GTPase, COPII associated small GTPase

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Product Information

83168-7-PBS targets SAR1A in IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: SAR1 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 22 kDa
• GenBank Accession Number: NM_020150
• Gene Symbol: SAR1A
• Gene ID (NCBI): 56681
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9NR31
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

SAR1A, also named as SAR1, SARA and SARA1, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1A is required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES).