CoraLite® Plus 488-conjugated SAFB2 Polyclonal antibody

SAFB2 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11642

Synonyms

SAF-B2, SAF B2, KIAA0138

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Validation Data Gallery

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  IF Staining of U2OS using CL488-11642

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 SAFB2 antibody (CL488-11642) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of U2OS using CL488-11642

  1x10^6 U2OS cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Safb2 Polyclonal Antibody (CL488-11642)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11642 targets SAFB2 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SAFB2 fusion protein Ag2230
• Full Name: scaffold attachment factor B2
• Calculated Molecular Weight: 528aa,57 kDa; 953aa,107 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC025279
• Gene Symbol: SAFB2
• Gene ID (NCBI): 9667
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SAFB2, also named as Scaffold attachment factor B2 or KIAA0138, is a 953 amino acid protein, which contains one SAP domain and one RRM (RNA recognition motif) domain. SAFB2 expressed at high levels in the CNS and at low levels in the liver. It also is expressed in a wide number of breast cancer cell lines. SAFB2 binds to scaffold/matrix attachment region (S/MAR) DNA and can function as an estrogen receptor corepressor and can also inhibit cell proliferation.