CoraLite® Plus 488-conjugated S6 Ribosomal protein Recombinant antibody

S6 Ribosomal protein Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse, zabrafish

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6L9

Cat no : CL488-80208

Synonyms

40S ribosomal protein S6, Phosphoprotein NP33, ribosomal protein S6, RPS6

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-80208

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 RPS6 antibody (CL488-80208, Clone: 6L9 ) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-80208 targets S6 Ribosomal protein in IF/ICC applications and shows reactivity with Human, rat, mouse, zabrafish samples.

• Tested Reactivity: Human, rat, mouse, zabrafish
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: ribosomal protein S6
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC000524
• Gene Symbol: RPS6
• Gene ID (NCBI): 6194
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.